Monoclonal Antibody to TLR2 (Clone: ABM3A87)

Fig-1: Western blot analysis of TLR2. Anti- TLR2 antibody (Clone: ABM3A87) was used at 2 µg/ml on mouse embryonic liver lysate.

Monoclonal Antibody to TLR2 (Clone: ABM3A87)

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Fig-1: Western blot analysis of TLR2. Anti- TLR2 antibody (Clone: ABM3A87) was used at 2 µg/ml on mouse embryonic liver lysate.

Fig-2 : Immunohistochemical analysis of TLR2 in human prostate tissue using TLR2 antibody (Clone: ABM3A87) at 5 µg/ml.

Fig-3: Intracellular flow analysis of TLR2 in PBMC (Monocytes) using 0.5 µg/10^6 cells of TLR2 antibody (Clone: ABM3A87). Green represents isotype control; red represents anti-TLR2 antibody. Goat anti-Mouse PE conjugate was used as secondary.

Fig-4: Intracellular flow analysis of TLR2 in THP-1 cells using 0.5 µg/10^6 cells of TLR2 antibody (Clone: ABM3A87). Green represents isotype control; red represents anti-TLR2 antibody. Goat anti-Mouse PE conjugate was used as secondary antibody.

Fig-5: Immunohistochemical analysis of TLR2 in Renal Cell Carcinoma using TLR2 antibody (Clone: ABM3A87) at 5 µg/ml.

Fig-6: Immunohistochemical analysis of TLR2 in human Colon tissue using TLR2 antibody (Clone: ABM3A87) at 10 µg/ml.

Product code: 10-3026

Clone name :	ABM3A87
Clonality :	Monoclonal
Application :	IHC, FACS, WB, IF
Reactivity :	Mouse, Human

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Download TDS MSDS

Format :	Purified
Amount :	100 µg
Isotype :	Mouse IgG1 Kappa
Purification :	Protein G Chromatography
Content :	25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	TLR2
Gene ID :	7097
Uniprot ID :	O60603
Alternative Name :	TLR2, TIL4
Immunogen Information :	A partial length recombinant TLR2 protein (amino acids 180-420) was used as the immunogen for this antibody.

TLR2 (Toll-Like Receptors 2) is a member of the TLR (Toll-like receptor) family that plays a fundamental role in pathogen recognition and activation of innate immunity. TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens. TLR2 also interacts with a large number of non-TLR molecules, allowing for recognition of a great number and variety of PAMPs (pathogen-associated molecular patterns). TLR2 expression has been detected in immune cells, endothelial, and epithelial cells.

Western blot analysis: 2-4 µg/ml, FACS analysis: 0.5 µg/10^6 cells, Immunohistochemical analysis: 5 µg/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

1. Parida R. Human MOSPD2: A bacterial Lmb mimicked auto-antigen is involved in immune infertility. Journal of Translational Autoimmunity. 2019 Apr;100002 (1). https://doi.org/10.1016/j.jtauto.2019.100002.(Immunofluorescence)

Subcellular location:	Membrane, Cytoplasmic vesicle, Membrane raft
Post transnational modification:	Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
Tissue Specificity:	Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
BioGrid:	112952. 27 interactions.